
September 17, 2022
Xeno-free induced pluripotent stem cell-derived neural progenitor cells for in vivo applications | Journal of Translational Medicine
Generation and maintenance of NPCs NPCs were generated as previously described with modifications. In detail, 80.000 iPSCs per well were plated on day − 2 on a 12-well plate, coated with Vitronectin (StemMACS iPS-Brew XF (Miltenyi), supplemented with 2uM Thiazovivin) and incubated overnight. On day − 1, cells were washed once in PBS and the medium was changed to fresh StemMACS iPS-Brew …